Fulvio Magni, Maciej Lalowski, Veronica Mainini, Martina Marchetti-Deschmann, Clizia Chinello, Andrea Urbani and Marc Baumann
Journal of Nephrology. 2013 May-Jun;26(3):430-436.
Matrix-assisted laser desorption/ionization (MALDI) and mass spectrometry (MS) imaging are advanced technologies capable of revealing the spatial distribution of different molecules–e.g., drugs and their metabolites, endogenous lipids and complex peptides/proteins–directly in tissue specimens at the same time. Information obtained regarding tissues by MALDI profiling/imaging analysis can be correlated with other MS-based techniques, auxiliary imaging technologies and routine immunohistochemical stainings. In this review we describe the MALDI profiling/imaging technologies, providing examples of their application in kidney research.